To a reflux apparatus add 4 g of piperine, 45 mL of methanol. 5 mL of water and 9 g of potassium hydroxide with stirring. Reflux for 24 h, cool down, filter and wash with some methanol. Dry in the open air. Then combine the product with 80ml of water and 10 mL of conc. HCl with stirring, cool down and filter. Recrystallize in hot ethanol.
The refluxed mixture, the mixture needs to be filtered and washed
Addition of the HCl
Filtration of the product
Samples of the pure product under normal and UV-light