Synthesis of D-Luciferin

110 mg of D-cysteine is dissolved in 2 mL of water, the solution is made alkaline with a solution of 0.17 g of sodium hydroxide in 1 mL of water. After deoxygenating the solution, the reaction vessel is wrapped with aluminum foil to protect the solution from exposure to light. Subsequently, 120 mg of 6-hydroxybenzothiazole-2-carbonitrile dissolved in 4.5 mL of methanol are added, the solution stirred for 1 h at room temperature and then the pH is adjusted to pH 2.5 with 2 M hydrochloric acid and allowed to stand for at least 20 h in a refrigerator. The precipitate is filtered and dried in a vacuum. The crude product is sufficiently pure, yet impurities are still present at this time, which should be separated by column chromatography.

Source

The solution

The mixture after acidification

Filtration

The crude product

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