Combine 25 g of glutamic acid with 60 mL of water, in a large beaker, start adding 13.4 g of ammonium bicarbonate slowly or a specific amount of ammonia in order to produce the corresponding ammonium salt. Start adding 300 mL of 6% hydrogen peroxide with stirring, the temperature must be below 80°C upon adding the hydrogen peroxide. After the bubbling stops start heating the solution to 85°C for an hour. Then evaporate to dryness and recrystallize in methanol or ethanol.
Adding ammonium bicarbonate
Adding hydrogen peroxide slowly
Keeping the solution below 80°C
Heating the solution to 85°C for 1h and evaporating it to dryness.